High-Sensitivity Flow Cytometry testing, focusing on high risk patients, ensures that PNH is diagnosed without delay4,5

PNH diagnosis is a matter of urgency1
PNH = Paroxysmal Nocturnal HaemoglobinuriaThrombosis is the leading cause of death in PNH1
High-sensitivity flow cytometry testing is essential in PNH diagnosis4
Granulocytes give the most accurate estimate of PNH clone size4,7,10
Analysis of granulocytes and RBCs in a patient with PNH4
PNH granulocyte clone: 55%
PNH RBC clone: 6%
In patients with an initial PNH clone size of 0.11% – 1% and 1.1% – 10%:
Adapted from Movalia et al, 2011.
Study design: An analysis of the incidence of PNH clones in 6897 patients recommended for testing according to the guidelines from the ICCS and IPIG. High Sensitivity Flow Cytometry analysis, with sensitivity up to 0.01% was used to screen patients for a PNH clone (421/6897 patients had a clone detected). Changes in PNH clone size over a period of 3–12 months were assessed in 89 PNH-positive patients who had follow-up data available.
89/421 patients had follow up studies assessed over a period of 3-12 months. 64/89 patients had initial PNH clone sizes of 0.01% – 0.1% or 10.1 – 100%; one patient had a change in clone size category. 25/89 patients had initial PNH clones 0.11% – 1% and 1.1% – 10%; 16/25 (64%) had changes in PNH clone size category and 9/25 (36%) had no change in PNH clone size category.
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References
1. Hillmen P, Muus P, Dührsen U, et al. Blood. 2007;110:4123-4128.
2. Brodsky RA. Blood Rev. 2008;22:65-74.
3. Lee JW, et al. Int J Hematol. 2013;97(6):749-757.
4. Borowitz MJ, et al; for Clinical Cytometry Society. Cytometry Part B. 2010;78B:211-230.
5. Morado M,et al. Cytometry Part B. 2017;92(5):361-370.
6. Movalia K, et al. Blood. 2011 118:1033.
7. Parker C, et al. for International PNH Interest Group. Blood. 2005;106:3699-3709.
8. Sutherland R, et al. Am J Clin Pathol. 2009;132:564-572.
9. Hochsmann B, et al. Ann Hematol. 2011;90:887-899.
10. Brodsky RA. Blood. 2009;113:6522-6527.